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px330 cas9  (Addgene inc)


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    Structured Review

    Addgene inc px330 cas9
    Px330 Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 3000 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px330+cas9+plasmid/pX330-U6-Chimeric_BB-CBh-hSpCas9+(Plasmid+%2342230)/pm41912567-249-19-20
    Average 96 stars, based on 3000 article reviews
    px330 cas9 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Distinct disease mutations in DNMT3A result in a spectrum of behavioral, epigenetic, and transcriptional deficits.
    Article Snippet: .. Cell Reports 42, 113411, November 28, 2023 19 sgRNAs were cloned into the pX330 Cas9 plasmid (Addgene), and then transfected into N2A cells. .. Validation was done using the T7 enzyme assay by the Washington University School of Medicine Transgenic Vectors Core. sgRNAs were transcribed in vitro using MEGAShortScript (Ambion), and Cas9 mRNA was in vitro transcribed, G-capped and poly-A tailed using the mMessageMachine kit (Ambion). mRNA of the sgRNA and Cas9 were then injected into hybrid C57Bl/6J x CBA fertilized eggs at the mouse genetics core at Washington University School of Medicine.

    Article Title: Regulation of kinesin-2 motility by its β-hairpin motif
    Article Snippet: .. For CRISPR–Cas9 genome editing in IMCD-3-FlpIn cells to generate a Kif3B KO, guide RNA (5′-AAGCTCAGAATCAGTCCGGG-3′) targeting exon 2 of Kif3B was designed in Benchling and cloned into the pX330 Cas9 plasmid (gift from F. Zhang; Addgene plasmid no. 42230) . .. IMCD-3 cells were transfected with 2.4 μg DNA (1.2 μg Kif3B guide expressing plasmid and 1.2 μg mScarlet vector) using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) in Opti-MEM reduced serum medium (Thermo Fisher Scientific) .

    Article Title: Distinct disease mutations in DNMT3A result in a spectrum of behavioral, epigenetic, and transcriptional deficits
    Article Snippet: .. This mutation changed the proline CCG codon into a leucine CTG codon ( ). sgRNAs were cloned into the pX330 Cas9 plasmid (Addgene), and then transfected into N2A cells. .. Validation was done using the T7 enzyme assay by the Washington University School of Medicine Transgenic Vectors Core. sgRNAs were transcribed in vitro using MEGAShortScript (Ambion), and Cas9 mRNA was in vitro transcribed, G-capped and poly-A tailed using the mMessageMachine kit (Ambion). mRNA of the sgRNA and Cas9 were then injected into hybrid C57Bl/6J x CBA fertilized eggs at the mouse genetics core at Washington University School of Medicine.

    Article Title: Beta-hairpin Mechanism of Autoinhibition and Activation in the Kinesin-2 Family
    Article Snippet: .. For CRISPR-Cas9 genome editing in IMCD-3-FlpIn cells to generate a Kif3B knockout, guide RNA 5’-AAGCTCAGAATCAGTCCGGG-3’ targeting exon 2 of Kif3B was designed in Benchling and cloned into the pX330 Cas9 plasmid (gift from Feng Zhang; Addgene plasmid #42230) . ..

    Article Title: Distinct disease mutations in DNMT3A result in a spectrum of behavioral, epigenetic, and transcriptional deficits
    Article Snippet: .. This mutation changed the proline CCG codon into a leucine CTG codon ( ). sgRNAs were cloned into the pX330 Cas9 plasmid (Addgene), and then transfected into N2A cells. .. Validation was done using the T7 enzyme assay by the Washington University School of Medicine Transgenic Vectors Core. sgRNAs were transcribed in vitro using MEGAShortScript (Ambion), and Cas9 mRNA was in vitro transcribed, G-capped and poly-A tailed using the mMessageMachine kit (Ambion). mRNA of the sgRNA and Cas9 were then injected into hybrid C57Bl/6J × CBA fertilized eggs at the mouse genetics core at Washington University School of Medicine.

    Article Title: Regulation of kinesin-2 motility by its β-hairpin motif.
    Article Snippet: .. For CRISPR–Cas9 genome editing in IMCD-3-FlpIn cells to generate a Kif3B KO, guide RNA (5′-AAGCTCAGAATCAGTCCGGG-3′) targeting exon 2 of Kif3B was designed in Benchling and cloned into the pX330 Cas9 plasmid (gift from F. Zhang; Addgene plasmid no. 42230)81. .. IMCD-3 cells were transfected with 2.4 μg DNA (1.2 μg Kif3B guide expressing plasmid and 1.2 μg mScarlet vector) using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) in Opti-MEM reduced serum medium (Thermo Fisher Scientific)69.

    Plasmid Preparation:

    Article Title: Distinct disease mutations in DNMT3A result in a spectrum of behavioral, epigenetic, and transcriptional deficits.
    Article Snippet: .. Cell Reports 42, 113411, November 28, 2023 19 sgRNAs were cloned into the pX330 Cas9 plasmid (Addgene), and then transfected into N2A cells. .. Validation was done using the T7 enzyme assay by the Washington University School of Medicine Transgenic Vectors Core. sgRNAs were transcribed in vitro using MEGAShortScript (Ambion), and Cas9 mRNA was in vitro transcribed, G-capped and poly-A tailed using the mMessageMachine kit (Ambion). mRNA of the sgRNA and Cas9 were then injected into hybrid C57Bl/6J x CBA fertilized eggs at the mouse genetics core at Washington University School of Medicine.

    Article Title: Regulation of kinesin-2 motility by its β-hairpin motif
    Article Snippet: .. For CRISPR–Cas9 genome editing in IMCD-3-FlpIn cells to generate a Kif3B KO, guide RNA (5′-AAGCTCAGAATCAGTCCGGG-3′) targeting exon 2 of Kif3B was designed in Benchling and cloned into the pX330 Cas9 plasmid (gift from F. Zhang; Addgene plasmid no. 42230) . .. IMCD-3 cells were transfected with 2.4 μg DNA (1.2 μg Kif3B guide expressing plasmid and 1.2 μg mScarlet vector) using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) in Opti-MEM reduced serum medium (Thermo Fisher Scientific) .

    Article Title: Distinct disease mutations in DNMT3A result in a spectrum of behavioral, epigenetic, and transcriptional deficits
    Article Snippet: .. This mutation changed the proline CCG codon into a leucine CTG codon ( ). sgRNAs were cloned into the pX330 Cas9 plasmid (Addgene), and then transfected into N2A cells. .. Validation was done using the T7 enzyme assay by the Washington University School of Medicine Transgenic Vectors Core. sgRNAs were transcribed in vitro using MEGAShortScript (Ambion), and Cas9 mRNA was in vitro transcribed, G-capped and poly-A tailed using the mMessageMachine kit (Ambion). mRNA of the sgRNA and Cas9 were then injected into hybrid C57Bl/6J x CBA fertilized eggs at the mouse genetics core at Washington University School of Medicine.

    Article Title: Beta-hairpin Mechanism of Autoinhibition and Activation in the Kinesin-2 Family
    Article Snippet: .. For CRISPR-Cas9 genome editing in IMCD-3-FlpIn cells to generate a Kif3B knockout, guide RNA 5’-AAGCTCAGAATCAGTCCGGG-3’ targeting exon 2 of Kif3B was designed in Benchling and cloned into the pX330 Cas9 plasmid (gift from Feng Zhang; Addgene plasmid #42230) . ..

    Article Title: USP7 represses lineage differentiation genes in mouse embryonic stem cells by both catalytic and noncatalytic activities
    Article Snippet: .. For gene knockout, mESCs were transfected with a px330-Cas9 plasmid (Addgene) together with a pair of sgRNAs targeting intronic sequences flanking exon1 of Usp7 gene using Lipofectamine 2000 (Life Technologies). ..

    Article Title: Distinct disease mutations in DNMT3A result in a spectrum of behavioral, epigenetic, and transcriptional deficits
    Article Snippet: .. This mutation changed the proline CCG codon into a leucine CTG codon ( ). sgRNAs were cloned into the pX330 Cas9 plasmid (Addgene), and then transfected into N2A cells. .. Validation was done using the T7 enzyme assay by the Washington University School of Medicine Transgenic Vectors Core. sgRNAs were transcribed in vitro using MEGAShortScript (Ambion), and Cas9 mRNA was in vitro transcribed, G-capped and poly-A tailed using the mMessageMachine kit (Ambion). mRNA of the sgRNA and Cas9 were then injected into hybrid C57Bl/6J × CBA fertilized eggs at the mouse genetics core at Washington University School of Medicine.

    Article Title: USP7 represses lineage differentiation genes in mouse embryonic stem cells by both catalytic and noncatalytic activities.
    Article Snippet: .. For gene knockout, mESCs were transfected with a px330-Cas9 plasmid (Addgene) together with a pair of sgRNAs targeting intronic sequences flanking exon1 of Usp7 gene using Lipofectamine 2000 (Life Technologies). ..

    Article Title: Regulation of kinesin-2 motility by its β-hairpin motif.
    Article Snippet: .. For CRISPR–Cas9 genome editing in IMCD-3-FlpIn cells to generate a Kif3B KO, guide RNA (5′-AAGCTCAGAATCAGTCCGGG-3′) targeting exon 2 of Kif3B was designed in Benchling and cloned into the pX330 Cas9 plasmid (gift from F. Zhang; Addgene plasmid no. 42230)81. .. IMCD-3 cells were transfected with 2.4 μg DNA (1.2 μg Kif3B guide expressing plasmid and 1.2 μg mScarlet vector) using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) in Opti-MEM reduced serum medium (Thermo Fisher Scientific)69.

    Transfection:

    Article Title: Distinct disease mutations in DNMT3A result in a spectrum of behavioral, epigenetic, and transcriptional deficits.
    Article Snippet: .. Cell Reports 42, 113411, November 28, 2023 19 sgRNAs were cloned into the pX330 Cas9 plasmid (Addgene), and then transfected into N2A cells. .. Validation was done using the T7 enzyme assay by the Washington University School of Medicine Transgenic Vectors Core. sgRNAs were transcribed in vitro using MEGAShortScript (Ambion), and Cas9 mRNA was in vitro transcribed, G-capped and poly-A tailed using the mMessageMachine kit (Ambion). mRNA of the sgRNA and Cas9 were then injected into hybrid C57Bl/6J x CBA fertilized eggs at the mouse genetics core at Washington University School of Medicine.

    Article Title: Distinct disease mutations in DNMT3A result in a spectrum of behavioral, epigenetic, and transcriptional deficits
    Article Snippet: .. This mutation changed the proline CCG codon into a leucine CTG codon ( ). sgRNAs were cloned into the pX330 Cas9 plasmid (Addgene), and then transfected into N2A cells. .. Validation was done using the T7 enzyme assay by the Washington University School of Medicine Transgenic Vectors Core. sgRNAs were transcribed in vitro using MEGAShortScript (Ambion), and Cas9 mRNA was in vitro transcribed, G-capped and poly-A tailed using the mMessageMachine kit (Ambion). mRNA of the sgRNA and Cas9 were then injected into hybrid C57Bl/6J x CBA fertilized eggs at the mouse genetics core at Washington University School of Medicine.

    Article Title: USP7 represses lineage differentiation genes in mouse embryonic stem cells by both catalytic and noncatalytic activities
    Article Snippet: .. For gene knockout, mESCs were transfected with a px330-Cas9 plasmid (Addgene) together with a pair of sgRNAs targeting intronic sequences flanking exon1 of Usp7 gene using Lipofectamine 2000 (Life Technologies). ..

    Article Title: Distinct disease mutations in DNMT3A result in a spectrum of behavioral, epigenetic, and transcriptional deficits
    Article Snippet: .. This mutation changed the proline CCG codon into a leucine CTG codon ( ). sgRNAs were cloned into the pX330 Cas9 plasmid (Addgene), and then transfected into N2A cells. .. Validation was done using the T7 enzyme assay by the Washington University School of Medicine Transgenic Vectors Core. sgRNAs were transcribed in vitro using MEGAShortScript (Ambion), and Cas9 mRNA was in vitro transcribed, G-capped and poly-A tailed using the mMessageMachine kit (Ambion). mRNA of the sgRNA and Cas9 were then injected into hybrid C57Bl/6J × CBA fertilized eggs at the mouse genetics core at Washington University School of Medicine.

    Article Title: USP7 represses lineage differentiation genes in mouse embryonic stem cells by both catalytic and noncatalytic activities.
    Article Snippet: .. For gene knockout, mESCs were transfected with a px330-Cas9 plasmid (Addgene) together with a pair of sgRNAs targeting intronic sequences flanking exon1 of Usp7 gene using Lipofectamine 2000 (Life Technologies). ..

    CRISPR:

    Article Title: Regulation of kinesin-2 motility by its β-hairpin motif
    Article Snippet: .. For CRISPR–Cas9 genome editing in IMCD-3-FlpIn cells to generate a Kif3B KO, guide RNA (5′-AAGCTCAGAATCAGTCCGGG-3′) targeting exon 2 of Kif3B was designed in Benchling and cloned into the pX330 Cas9 plasmid (gift from F. Zhang; Addgene plasmid no. 42230) . .. IMCD-3 cells were transfected with 2.4 μg DNA (1.2 μg Kif3B guide expressing plasmid and 1.2 μg mScarlet vector) using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) in Opti-MEM reduced serum medium (Thermo Fisher Scientific) .

    Article Title: Beta-hairpin Mechanism of Autoinhibition and Activation in the Kinesin-2 Family
    Article Snippet: .. For CRISPR-Cas9 genome editing in IMCD-3-FlpIn cells to generate a Kif3B knockout, guide RNA 5’-AAGCTCAGAATCAGTCCGGG-3’ targeting exon 2 of Kif3B was designed in Benchling and cloned into the pX330 Cas9 plasmid (gift from Feng Zhang; Addgene plasmid #42230) . ..

    Article Title: Regulation of kinesin-2 motility by its β-hairpin motif.
    Article Snippet: .. For CRISPR–Cas9 genome editing in IMCD-3-FlpIn cells to generate a Kif3B KO, guide RNA (5′-AAGCTCAGAATCAGTCCGGG-3′) targeting exon 2 of Kif3B was designed in Benchling and cloned into the pX330 Cas9 plasmid (gift from F. Zhang; Addgene plasmid no. 42230)81. .. IMCD-3 cells were transfected with 2.4 μg DNA (1.2 μg Kif3B guide expressing plasmid and 1.2 μg mScarlet vector) using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) in Opti-MEM reduced serum medium (Thermo Fisher Scientific)69.

    Mutagenesis:

    Article Title: Distinct disease mutations in DNMT3A result in a spectrum of behavioral, epigenetic, and transcriptional deficits
    Article Snippet: .. This mutation changed the proline CCG codon into a leucine CTG codon ( ). sgRNAs were cloned into the pX330 Cas9 plasmid (Addgene), and then transfected into N2A cells. .. Validation was done using the T7 enzyme assay by the Washington University School of Medicine Transgenic Vectors Core. sgRNAs were transcribed in vitro using MEGAShortScript (Ambion), and Cas9 mRNA was in vitro transcribed, G-capped and poly-A tailed using the mMessageMachine kit (Ambion). mRNA of the sgRNA and Cas9 were then injected into hybrid C57Bl/6J x CBA fertilized eggs at the mouse genetics core at Washington University School of Medicine.

    Article Title: Distinct disease mutations in DNMT3A result in a spectrum of behavioral, epigenetic, and transcriptional deficits
    Article Snippet: .. This mutation changed the proline CCG codon into a leucine CTG codon ( ). sgRNAs were cloned into the pX330 Cas9 plasmid (Addgene), and then transfected into N2A cells. .. Validation was done using the T7 enzyme assay by the Washington University School of Medicine Transgenic Vectors Core. sgRNAs were transcribed in vitro using MEGAShortScript (Ambion), and Cas9 mRNA was in vitro transcribed, G-capped and poly-A tailed using the mMessageMachine kit (Ambion). mRNA of the sgRNA and Cas9 were then injected into hybrid C57Bl/6J × CBA fertilized eggs at the mouse genetics core at Washington University School of Medicine.

    Knock-Out:

    Article Title: Beta-hairpin Mechanism of Autoinhibition and Activation in the Kinesin-2 Family
    Article Snippet: .. For CRISPR-Cas9 genome editing in IMCD-3-FlpIn cells to generate a Kif3B knockout, guide RNA 5’-AAGCTCAGAATCAGTCCGGG-3’ targeting exon 2 of Kif3B was designed in Benchling and cloned into the pX330 Cas9 plasmid (gift from Feng Zhang; Addgene plasmid #42230) . ..

    Gene Knockout:

    Article Title: USP7 represses lineage differentiation genes in mouse embryonic stem cells by both catalytic and noncatalytic activities
    Article Snippet: .. For gene knockout, mESCs were transfected with a px330-Cas9 plasmid (Addgene) together with a pair of sgRNAs targeting intronic sequences flanking exon1 of Usp7 gene using Lipofectamine 2000 (Life Technologies). ..

    Article Title: USP7 represses lineage differentiation genes in mouse embryonic stem cells by both catalytic and noncatalytic activities.
    Article Snippet: .. For gene knockout, mESCs were transfected with a px330-Cas9 plasmid (Addgene) together with a pair of sgRNAs targeting intronic sequences flanking exon1 of Usp7 gene using Lipofectamine 2000 (Life Technologies). ..



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